WHAT IS BRUCELLOSIS AGGLUTININS TEST?
Brucellosis (Bang’s disease, Malta fever, Mediterranean fever, undulant fever) is a systemic disease acquired from animals that lasts days to years. It is found with the greatest frequency in Europe, North Africa, Asia, Mexico, and South America. Brucella is an obligate parasite on animals. The mode of transmission to humans is through direct body tissue contact with fluids, milk, and dairy products of infected animals or by transmission to infants by breastfeeding. Onset may be acute or insidious, and symptoms may include arthralgia, body aches, chills, diaphoresis, depression, fever(s), headache, weakness, pneumonitis, and nonpurulent meningitis. In this test, Brucella antigens are mixed with a client’s serum and observed for an agglutination reaction. The sample is heated and observed for clumping and unclumping. A sample that clumps upon warming and unclumps upon cooling is considered a positive test. A positive reaction is followed by serial dilutions of serum and retesting. The results are expressed as the highest titer showing agglutination. Agglutination at a titer greater than 1 : 80 indicates the presence of antibodies generated by any of three closely related Brucella species and is used in the indirect diagnosis of human brucellosis.